PMEL CLIA Kit for Chicken(Gallus gallus) | EIAab.comPMEL CLIA Kit for Chicken(Gallus gallus) | EIAab.com

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PMEL (Gene name),

Melanocyte protein PMEL (Protein name ), PMEL_CHICK from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

PMEL(MMP115;PMEL17;SILV);

Protein name:

Melanocyte protein PMEL;

Alternative:

Melanocyte protein Pmel 17;115 kDa melanosomal matrix protein;Silver locus protein homolog;Premelanosome protein;

Organism:

Chicken (Gallus gallus).

General Annotation

Sub Unit:

N/A

Function:

Might be required for polymerization of melanin onto the core structure of melanosomes with enzymic function of tyrosinase.

Subcellular Location:

Melanosome lumen On the fibrous matrix structure of the premelanosome.

Protein Attributes:

Sequence length:

763

Sequence:

50:

MRLHGAIVLL | AALLALVTAQ | QRGGGRSRGG | VKGSAWGGRP | APFRSWDTAR |

100:

YRPWQEGTAR | QNDCWRGGDV | TFDISNDAPT | LVGARATFSI | ALRFPGTQTV |

150:

LPDGRVVWSQ | NCTVNGTRML | QGDPVYPEQL | AEGSDGVFPD | GQPFPRSAWG |

200:

KRGRFVYVWW | TWGRYWQVVD | GATSQLTVGT | DGVALGSYTM | EVVVYHYRGR |

250:

QRFIPIGHAS | TQFSITDQVP | IAVDVTQLEV | AAGDGGSFVR | NRPVAFNVRL |

300:

HDPSHYLRDA | DISYSWDFGD | QSGTLISRSP | TVTHTYLQAG | SFAARLVLQA |

350:

AIPLSSCGTS | APPVVDPTTG | PVPSLGPTAT | QPVGPTGSGT | ATAPSNLTGS |

400:

GTAAAPGTTA | APRASGAPAE | PTGVSVAVLS | DSAATEPLPD | PVLSTAVADA |

450:

AAGTDPTADP | LPPTSVSSGG | DAPGTVAPTA | VEGSVAAGVG | TAEDVAAATP |

500:

GATAADVAVD | TAGATDGDAV | GPTAAATAES | IADPTAGATD | GDAVGATAES |

550:

IADPTAGATD | GDAVGPTAAA | TAESIADPTA | GATAVSSGSA | TAGATAEPLL |

600:

LVKRQAPEAE | PTGCVLYRYG | TFSTELNIVQ | GIESVAIVQV | VPAAPEGSGN |

650:

SVELTVTCEG | SLPEEVCTVV | ADAECRTAQM | QTCSAVAPAP | GCQLVLRQDF |

700:

NQSGLYCLNV | SLANGNGLAV | ASTHVAVGGA | SPAASGTTLT | VGLLWAPLIA |

750:

AALGTAAYTY | RRVKYSPLLP | TAPTAPRPHS | WLPPGATLRL | LLRQAFGGAP |

763:

SGESSPLLRA | NAV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Pfam:

STRING:

9031.ENSGALP00000023540

String:

KEGG:

Uniprot:

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ELISA Kit for Chicken Melanocyte protein PMEL

Cat.:

E2299m

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Packing:

96T

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ELISA Kit for Chicken Melanocyte protein PMEL

Cat.:

E2299h

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96T

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ELISA Kit for Chicken Melanocyte protein PMEL

Cat.:

E2299c

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Packing:

96T

ELISA Kit for Chicken Melanocyte protein PMEL

Cat.:

E2299b

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Packing:

96T

CLIA Kit for Chicken Melanocyte protein PMEL

Cat.:

U2299c

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MSDS:

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Packing:

96T

CLIA Kit for Chicken Melanocyte protein PMEL

Cat.:

U2299m

Price:

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MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Chicken Melanocyte protein PMEL

Cat.:

U2299h

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

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CLIA Kit for Chicken Melanocyte protein PMEL

Cat.:

U2299b

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MSDS:

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Packing:

96T

Polyclonal Antibody for Chicken Melanocyte protein PMEL

Polyclonal Antibody for Chicken Melanocyte protein PMEL

Cat.:

P2299Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Chicken Melanocyte protein PMEL

Cat.:

P2299Rb-m

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Chicken Melanocyte protein PMEL

Monoclonal Antibody for Chicken Melanocyte protein PMEL

Monoclonal Antibody for Chicken Melanocyte protein PMEL

Monoclonal Antibody for Chicken Melanocyte protein PMEL

Monoclonal Antibody for Chicken Melanocyte protein PMEL

Protein for Chicken Melanocyte protein PMEL

Protein for Chicken Melanocyte protein PMEL

Protein for Chicken Melanocyte protein PMEL

Protein for Chicken Melanocyte protein PMEL

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.1%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

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Recovery was determined by spiking various levels of PMEL into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	108	105-113
Plasma	107	104-112

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PMEL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	104-110%	102-113%	100-116%	104-114%
EDTA plasma(n=5)	105-110%	98-116%	104-112%	102-112%
heparin plasma(n=5)	100-114%	103-112%	100-115%	101-113%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"Complete sequence and expression of a cDNA encoding a chicken 115-kDa melanosomal matrix protein."
Mochii M. , Agata K. , Eguchi G.
Pigment Cell Res.4:41-47(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: White leghorn.
tissue: Retinal pigment epithelium.
2.

Mochii M. , Agata K. , Eguchi G. , Zhong J. , Kuliawat R.
Submitted (2004-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 383-384 AND 679-758
3.

"The Dominant white, Dun and Smoky color variants in chicken are associated with insertion/deletion polymorphisms in the PMEL17 gene."
Kerje S. , Sharma P. , Gunnarsson U. , Kim H. , Bagchi S. , Fredriksson R. , Schuetz K. , Jensen P. , von Heijne G. , Okimoto R. , Andersson L.
Genetics168:1507-1518(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: Black Langshan.
strain: Broiler.
strain: Dun.
strain: Red jungle fowl.
strain: Smoky.
strain: White leghorn.
4.

"Expression of gene coding for a melanosomal matrix protein transcriptionally regulated in the transdifferentiation of chick embryo pigmented epithelial cells."
Mochii M. , Agata K. , Kobayashi H. , Yamamoto T.S. , Eguchi G.
Cell Differ.24:67-74(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
strain: White leghorn.
tissue: Retinal pigment epithelium.

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